Comparative analysis of immunocritical melanoma markers in the mouse melanoma cell lines B16, K1735 and S91-M3

Peter, I Mezzacasa, A LeDonne, P Dummer, R Hemmi, S

I. Peter et al., Comparative analysis of immunocritical melanoma markers in the mouse melanoma cell lines B16, K1735 and S91-M3, MELANOMA RE, 11(1), 2001, pp. 21-30

46

INGLESE

Article

Oncology,"Onconogenesis & Cancer Research

MELANOMA RESEARCH

0960-8931 → ACNP

11

1

2001

21 - 30

ISI

0960-8931(200102)11:1<21:CAOIMM>2.0.ZU;2-N

The mouse melanoma cell lines B16, K1735 and Cloudman S91-M3 (and various s ublines) are frequently used as melanoma models. Extensive comparative data of their immunological features are not available. In order to define the immunological profiles of these cell lines, relevant tumour markers were st udied. S91-M3 melanoma cells constitutively expressed high levels of major histocompatibility complex (MHC) I, in contrast to K1735-M2 and B16-F1 cell s. MHC II expression was restricted to B16-F1 cells following interferon-ga mma treatment. Tyrosinase, tyrosinase-related protein-2 and gp100 were dete cted in B16-F1 and S91-M3 cells, but not in K1735-M2 cells. Constitutive su rface expression and secretion of intercellular adhesion molecule-1 was fou nd on S91-M3 cells. No substantial secretion of interleukin-10 could be det ected. In contrast, low levels of latent transforming growth factor-beta we re found in the cell supernatants of B16-F1 and K1735-M2 cells. The express ion pattern of Fas, FasL and FLICE inhibitory protein was comparable in all three cell lines. Thus our findings indicate that each cell line presents a characteristic immunological profile, confirming that B16-F1 is an approp riate murine tumour model for tumours with low levels of MHC I but expressi ng melanoma-associated antigens. S91-M3 represents a complementary, more im munogenic model. In contrast, K1735-M2 does not seem to be an appropriate m odel for melanoma. (C) 2001 Lippincott Williams & Wilkins.