To detect the molecules that interact with infectious bursal disease virus
(IBDV), the chicken B lymphoblastoid cell line, LSCC-BK3, which is permissi
ve for virulent IBDV infection was investigated. The sodium dodecyl sulfate
-solubilized plasma membrane fraction from the cells was subjected to a vir
us overlay protein binding assay. The IBDV specifically bound to proteins i
n LSCC-BK3 plasma membranes with molecular weights of 70, 82 and 110 kDa. T
his is the first report to demonstrate cellular molecules that interact wit
h virulent IBDV.