Complete nucleotide sequence of the cytochrome b gene of channel catfish Ictalurus punctatus and comparison of sequence homology among channel catfish and other fishes

Citation
T. Matsuo et al., Complete nucleotide sequence of the cytochrome b gene of channel catfish Ictalurus punctatus and comparison of sequence homology among channel catfish and other fishes, J VET MED S, 63(2), 2001, pp. 207-210
Citations number
9
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY MEDICAL SCIENCE
ISSN journal
0916-7250 → ACNP
Volume
63
Issue
2
Year of publication
2001
Pages
207 - 210
Database
ISI
SICI code
0916-7250(200102)63:2<207:CNSOTC>2.0.ZU;2-P
Abstract
To clarify the phylogenic relationship of channel catfish with other fishes , the cytochrome b (Cyt b) gene of the catfish was cloned and sequenced. Ch annel catfish (Ictalurus punctatus) belonging to the family Ictaluridae in the order Siluriformes showed a 78.4-87.4 % similarity to all but one fish of the family Cyprinidae and river leach Crossostoma lacustre of the family Balitoridae in the order Cypriniformes in which genes had already been seq uenced, and a 97.2% similarity to the goldfish (Carassius auratus) belongin g to the family Cyprinidae. Within the family Cyprinidae, a 78.8-89.2% simi larity to one another was recorded. In addition, the similarity rate betwee n the family Cyprinidae and the family Balitoridae reached a value of 77.8- 79.9% in the order Cypriniformes. Furthermore, in an unrooted phylogenetic tree consisting of four branches among eight fishes, channel catfish and go ldfish appeared in the same branch. These results suggested that the Cyt b gene of the channel catfish in the order Siluriformes was closely related t o that of a goldfish in the order Cypriniformes. The results were not agree ment with the morphological classification. Genetic reclassification of the fishes may be necessary to identify the ancestor. This is the first report on the cloning and complete sequencing the Cyt b gene of the channel catfi sh which may contribute to the genetic reclassification of catfishes belong ing to the order Siluriformes.