Isolation and characterization of the genes up-regulated in isolated neurons by aged garlic extract (AGE)

Citation
S. Sumi et al., Isolation and characterization of the genes up-regulated in isolated neurons by aged garlic extract (AGE), J NUTR, 131, 2001, pp. 1096S-1099S
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Food Science/Nutrition","Endocrinology, Nutrition & Metabolism
Journal title
JOURNAL OF NUTRITION
ISSN journal
0022-3166 → ACNP
Volume
131
Year of publication
2001
Supplement
3
Pages
1096S - 1099S
Database
ISI
SICI code
0022-3166(200103)131:<1096S:IACOTG>2.0.ZU;2-T
Abstract
Aged garlic extract (AGE) produces neurotrophic effects on cultured fetal r at hippocampal neurons. These studies examined the molecular events trigger ed by AGE that might account for a suppression of neuronal cell death. Gene s differentially expressed by the addition of AGE in primary cultured hippo campal neurons isolated from fetal rat brain were screened using mRNA diffe rential display. Four cDNA clones were significantly enhanced at their tran scriptional level; they were designated as #24, #110, #153 and #155. Quanti tative reverse transcription-polymerase chain reaction (RT-PCR), as well as dot-blot hybridization combined with RT-PCR, confirmed that the transcript ion from these four genes was elevated at least twofold, particularly the m RNA of #153, which was increased >20 times 72 h after the addition of AGE. A homology search of the respective cDNA sequences in the DNA database reve aled that #153 is an alpha2-microglobulin-related protein (alpha 2MRP) gene . The others genes were not identified. Induction of the alpha 2MRP gene ex pression occurred within 24 h after addition of AGE. These findings suggest a possible mechanism by which AGE may regulate gene expression and bring a bout a neurotrophic effect. Further, our results suggest that alpha 2MRP ma y function at the initial step of the molecular events triggered by AGE and play an important role in the survival of hippocampal neurons.