Diversity within reference strains of Corynebacterium matruchotii includesCorynebacterium durum and a novel organism

Barrett, SLR Cookson, BT Carlson, LC Bernard, KA Coyle, MB

Slr. Barrett et al., Diversity within reference strains of Corynebacterium matruchotii includesCorynebacterium durum and a novel organism, J CLIN MICR, 39(3), 2001, pp. 943-948

27

INGLESE

Article

Clinical Immunolgy & Infectious Disease",Microbiology

JOURNAL OF CLINICAL MICROBIOLOGY

0095-1137 → ACNP

39

3

2001

943 - 948

ISI

0095-1137(200103)39:3<943:DWRSOC>2.0.ZU;2-F

Corynebacterium matruchotii has been the subject of numerous dental pathoge nesis studies. The purpose of the present study was to resolve concerns abo ut diversity within the reference strains of C. matruchotii through analysi s of seven strains procured from the American Type Culture Collection (ATCC ). Analysis of whole-cell fatty acid profiles with the library generation s oftware of Microbial ID Inc. revealed that three types of organisms have be en deposited in the ATCC as C, matruchotii. These three groups of organisms were also distinguishable by DNA-DNA dot blot hybridization, by sequences of two hypervariable regions of the 16S rRNA gene, and by the pyrrolidonyl arylamidase test. These studies indicate that two C. matruchotii reference strains, ATCC 33449 and ATCC 33822, are members of the recently proposed sp ecies, Corynebacterium durum. The colonial morphology and biochemical react ions of the C, durum strains are more diverse than originally reported. Str ain ATCC 43833 is unique and represents a novel species. In addition to the type strain, ATCC 14266, true members of the species C, matruchotii includ e ATCC strains 14265, 33806, and 43832 plus two reference strains, L2 and R ichardson 13, which comprise the vast majority of strains used in dental pa thogenesis research with this species.