Tissue distribution and temperature-dependence of Anguilla anguilla L. EROD activity following exposure to model inducers and relationship with plasma cortisol, lactate and glucose levels

Citation
M. Pacheco et Ma. Santos, Tissue distribution and temperature-dependence of Anguilla anguilla L. EROD activity following exposure to model inducers and relationship with plasma cortisol, lactate and glucose levels, ENVIRON INT, 26(3), 2001, pp. 149-155
Citations number
62
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Environment/Ecology
Journal title
ENVIRONMENT INTERNATIONAL
ISSN journal
0160-4120 → ACNP
Volume
26
Issue
3
Year of publication
2001
Pages
149 - 155
Database
ISI
SICI code
0160-4120(200101)26:3<149:TDATOA>2.0.ZU;2-W
Abstract
Anguilla anguilla L. ethoxyresorufin-O-deethylase (EROD) elevation by 2.7 m uM beta -naphthoflavone (BNF) 3 days water exposure, or 4 mg/kg ip exposure , was studied in four different organs - liver, kidney, gills, and intestin e. The results demonstrated a significant increase in liver EROD activity f or the two previous conditions, whereas kidney EROD activity only increased during the intraperitoneal exposure. A. anguilla was also exposed during 8 , 16, and 24 h to water contaminated with 2.7 muM BNF or benzo[a]pyrene (Ba P) at 20 degreesC and 25 degreesC. Both compounds significantly increased l iver EROD activity from 8 up to 24 h. There was no significant difference i n liver EROD activity elevation by both compounds, either at 20 degreesC or 25 degreesC. Liver EROD activity was demonstrated to be one of the first w arning systems concerning the presence of polycyclic aromatic hydrocarbons (PAHs) in water. A. anguilla 3 h exposure to diesel oil water-soluble fract ion (DWSF) significantly increased plasma cortisol and significantly decrea sed plasma lactate. A prolonged exposure beyond 3 h, i.e. 4 h, 2, 3, 4, and 6 days to the previous conditions demonstrated a significant liver EROD ac tivity elevation from Day 2 up to 6, and a significant increase in erythroc ytic nuclear abnormalities (ENA) at Day 6. (C) 2001 Elsevier Science Ltd. A ll rights reserved.