Cimetidine inhibits production of interferon gamma and tumor necrosis factor alpha by splenocytes in aplastic anemic mice

Citation
T. Xia et al., Cimetidine inhibits production of interferon gamma and tumor necrosis factor alpha by splenocytes in aplastic anemic mice, ACT PHAR SI, 22(3), 2001, pp. 239-242
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ACTA PHARMACOLOGICA SINICA
ISSN journal
0253-9756 → ACNP
Volume
22
Issue
3
Year of publication
2001
Pages
239 - 242
Database
ISI
SICI code
0253-9756(200103)22:3<239:CIPOIG>2.0.ZU;2-C
Abstract
AIM: To study the effects of cimetidine (Cim) on the production of interfer on gamma (IFN gamma) and tumor necrosis factor alpha (TNF alpha) by splenoc ytes in immune-derived aplastic anemic (AA) mice. METHODS: Aplastic anemic mice model was constructed first, and then the splenocytes were induced to secrete IFN gamma and TNF alpha. Concentration of IFN gamma was assayed usi ng sandwich ELISA, while that of TNF alpha was measured with L929 cytotoxic ity methods. RESULTS: (1) Concentrations of IFN gamma and TNF alpha secrete d by splenocytes from AA mice were (137 +/- 36) ng/L and (6 +/- 3) mug/L, r espectively, much more than the irradiated and the control mice. (2) Treatm ent with Cim 10 mu mol/L reduced the concentrations of IFN gamma and TNF al pha to (14 +/- 8) ng/L and (2.7 +/- 0.6) mug/L, respectively. CONCLUSION: C im could effectively reduce the production of IFN gamma and TNF alpha from splenocytes of AA mice.