Cimetidine inhibits production of interferon gamma and tumor necrosis factor alpha by splenocytes in aplastic anemic mice

Xia, T Wang, QR Xu, YH

T. Xia et al., Cimetidine inhibits production of interferon gamma and tumor necrosis factor alpha by splenocytes in aplastic anemic mice, ACT PHAR SI, 22(3), 2001, pp. 239-242

11

INGLESE

Article

Pharmacology & Toxicology

ACTA PHARMACOLOGICA SINICA

0253-9756 → ACNP

22

3

2001

239 - 242

ISI

0253-9756(200103)22:3<239:CIPOIG>2.0.ZU;2-C

AIM: To study the effects of cimetidine (Cim) on the production of interfer on gamma (IFN gamma) and tumor necrosis factor alpha (TNF alpha) by splenoc ytes in immune-derived aplastic anemic (AA) mice. METHODS: Aplastic anemic mice model was constructed first, and then the splenocytes were induced to secrete IFN gamma and TNF alpha. Concentration of IFN gamma was assayed usi ng sandwich ELISA, while that of TNF alpha was measured with L929 cytotoxic ity methods. RESULTS: (1) Concentrations of IFN gamma and TNF alpha secrete d by splenocytes from AA mice were (137 +/- 36) ng/L and (6 +/- 3) mug/L, r espectively, much more than the irradiated and the control mice. (2) Treatm ent with Cim 10 mu mol/L reduced the concentrations of IFN gamma and TNF al pha to (14 +/- 8) ng/L and (2.7 +/- 0.6) mug/L, respectively. CONCLUSION: C im could effectively reduce the production of IFN gamma and TNF alpha from splenocytes of AA mice.