Prostate short-chain dehydrogenase reductase 1 (PSDR1): A new member of the short-chain steroid dehydrogenase/reductase family highly expressed in normal and neoplastic prostate epithelium

Citation
By. Lin et al., Prostate short-chain dehydrogenase reductase 1 (PSDR1): A new member of the short-chain steroid dehydrogenase/reductase family highly expressed in normal and neoplastic prostate epithelium, CANCER RES, 61(4), 2001, pp. 1611-1618
Citations number
56
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER RESEARCH
ISSN journal
0008-5472 → ACNP
Volume
61
Issue
4
Year of publication
2001
Pages
1611 - 1618
Database
ISI
SICI code
0008-5472(20010215)61:4<1611:PSDR1(>2.0.ZU;2-W
Abstract
Genes regulated by androgenic hormones are of critical importance for the n ormal physiological function of the human prostate gland, and they contribu te to the development and progression of prostate carcinoma. We used cDNA m icroarrays comprised of prostate-derived cDNAs to profile transcripts regul ated by androgens in prostate cancer cells, This study identified a novel g ene that we have designated prostate short-chain dehydrogenase/reductase 1 (PSDR1), that exhibits increased expression on exposure to androgens in the LNCaP prostate cancer cell line. Northern analysis demonstrated that PSDR1 is highly expressed in the prostate gland relative to other normal human t issues. The PSDR1 cDNA and putative protein exhibit homology to the family of short-chain dehgydrogenase/reductase enzymes and thus identify a new mem ber of this family. Cloning and analysis of the putative PSDR1 promoter reg ion identified a potential androgen-response element. We used a radiation-h ybrid panel to map the PSDR1 gene to chromosome 14q23-24.3. In situ hybridi zation localizes PSDR1 expression to normal and neoplastic prostate epithel ium, These results identify a new gene involved in the androgen receptor-re gulated gene network of the human prostate that may play a role in the path ogenesis of prostate carcinoma.