Spatial arrangements and associative behavior of species in an in vitro oral biofilm model

Citation
M. Guggenheim et al., Spatial arrangements and associative behavior of species in an in vitro oral biofilm model, APPL ENVIR, 67(3), 2001, pp. 1343-1350
Citations number
56
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
0099-2240 → ACNP
Volume
67
Issue
3
Year of publication
2001
Pages
1343 - 1350
Database
ISI
SICI code
0099-2240(200103)67:3<1343:SAAABO>2.0.ZU;2-Q
Abstract
The spatial arrangements and associative behavior of Actinomyces naeslundii , Veillonella dispar, Fusobacteridum nucleatum, Streptococcus sobrinus, and Streptococcus oralis strains in an in vitro model of supragingival plaque were determined, Using species-specific fluorescence-labeled antibodies in conjunction with confocal laser scanning microscopy, the volumes and distri bution of the five strains were assessed during biofilm formation. The volu me derived cell numbers of each strain correlated well with respective cult ure data. Between 15 min and 64 h, populations of each strain increased in a manner reminiscent of batch growth. The microcolony morphologies of all m embers of the consortium and their distributions within the biofilm were ch aracterized, as were interspecies associations. Biofilms formed 15 min afte r inoculation consisted principally of single nonaggregated cells. All five strains adhered strongly to the saliva-conditioned substratum, and therefo re, coadhesion played no role during the initial phase of biofilm formation . This observation does not reflect the results of in vitro coaggregation o f the five strains, which depended upon the nature of the suspension medium . While the possibility cannot be excluded that some interspecies associati ons observed at later stages of biofilm formation were initiated by coadhes ion, increase in bacterial numbers appeared to be largely a growth phenomen on regulated by the prevailing cultivation conditions.