Expression of the adhesion molecules Mac-1 and L-selectin on neutrophils in acute pancreatitis is protease- and complement-dependent

Citation
W. Hartwig et al., Expression of the adhesion molecules Mac-1 and L-selectin on neutrophils in acute pancreatitis is protease- and complement-dependent, ANN SURG, 233(3), 2001, pp. 371-378
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Surgery,"Medical Research Diagnosis & Treatment
Journal title
ANNALS OF SURGERY
ISSN journal
0003-4932 → ACNP
Volume
233
Issue
3
Year of publication
2001
Pages
371 - 378
Database
ISI
SICI code
0003-4932(200103)233:3<371:EOTAMM>2.0.ZU;2-Z
Abstract
Objective To examine the effect of pancreatic proteases on the expression o f the adhesion molecules Mac-1 and L-selectin on neutrophils, and the role of complement activation in this process. Summary Background Data Sequestration of neutrophils in the pancreatic and pulmonary microvasculature characterizes acute pancreatitis. Methods Serum was collected from inbred rats after induction of necrotizing pancreatitis; trypsinogen activation peptide was measured to quantify tryp sin activation. Normal rat serum was also collected and subjected to limite d trypsin digestion with and without the addition of complement inhibitor. Both groups of sera were incubated in vitro with healthy leukocytes. Expres sion of Mac-1 and L-selectin on neutrophils was measured quantitatively by flow cyometry. To assess the consequences of these events in vivo, trypsina ted serum with or without complement inhibition or control serum was infuse d intravenous into rats. Soybean trypsin inhibitor was added to serum befor e injections to block residual trypsin activity. Pancreatic and pulmonary i njury was quantitiated by histology, measurement of edema, and myeloperoxid ase activity. Results Mac-1 expression on neutrophils incubated with pancreatitis serum w as increased compared with controls, whereas L-selectin was decreased. Neut rophils incubated with trypsinated serum also showed upregulation of Mac-1 and downregulation of L-selectin, particularly with trypsin at 10(-4) mol/L . Addition of soluble complement receptor 1 abrogated both Mac-1 upregulati on and L-selectin downregulation. Lungs of animals injected with trypsinate d serum showed increased edema and myeloperoxidase activity, which were red uced by soluble complement receptor 1. Conclusions Trypsin-generated complement activation participates in the upr egulation of Mac-1 and shedding of L-selectin on neutrophils in acute pancr eatitis. Protease or complement inhibition may be effective in preventing l eukocyte migration and subsequent local and remote organ injury.