Antioxidants vitamin C and dithiothreitol, activate membrane-bound guanylate cyclase in PC12 cells

Citation
Zj. Chen et al., Antioxidants vitamin C and dithiothreitol, activate membrane-bound guanylate cyclase in PC12 cells, J PHARM PHA, 53(2), 2001, pp. 243-247
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
JOURNAL OF PHARMACY AND PHARMACOLOGY
ISSN journal
0022-3573 → ACNP
Volume
53
Issue
2
Year of publication
2001
Pages
243 - 247
Database
ISI
SICI code
0022-3573(200102)53:2<243:AVCADA>2.0.ZU;2-5
Abstract
Antioxidants and antioxidant enzymes are known to protect against cell deat h induced by reactive oxygen species. However, apart from directly quenchin g free radicals, little is known about the effect of antioxidants on hormon e-activated second messenger systems. We previously found that antioxidants such as 17-beta estradiol and resveratrol activate membrane-bound guanylat e cyclase CC-A, the receptor for atrial natriuretic factor (ANF), in PC12 c ells. It is possible that other antioxidants may also activate membrane-bou nd guanylate cyclase CC-A. The aim of this study was to determine if dithio threitol (DTT), vitamin C, and vitamin E activate membrane-bound guanylate cyclase GC-A in PC12 cells. The results showed that both DTT and vitamin C increased cGMP levels in PC12 cells, whereas vitamin E had no effect. DTT a nd Vitamin C inhibited membrane-bound guanylate cyclase activity stimulated by ANF in PC12 cells. In contrast, DTT and Vitamin C had no effect on solu ble guanylate cyclase activity stimulated by substance P. Furthermore, NO s ynthase inhibitors L-NAME and aminoguanidine did not affect DTT- and vitami n C-stimulated guanylate cyclase activity. The results indicate that DTT an d vitamin C, but not vitamin E, activate membrane-bound guanylate cyclase G C-A in PC12 cells.