H. Harada et al., Molecular cloning, tissue expression, and chromosomal assignment of a novel gene encoding a subunit of the human signal-recognition particle, J HUM GENET, 46(2), 2001, pp. 70-75
Human cancers derived from breast, esophageal, or ovarian tissues frequentl
y show allelic losses on chromosome band 17q25. Moreover, a locus responsib
le for hereditary focal nonepidermolytic palmoplantar keratoderma, a condit
ion associated with esophageal cancer (TOC; tylosis with oesophageal cancer
), has been mapped to the same band. During efforts to sequence, by shotgun
methods, a 1-Mb target region that we had defined as the DNA segment harbo
ring the putative tumor suppressor gene(s) involved in these events, we ide
ntified a novel cDNA. The full-length cDNA is 2495bp long and is expressed
predominantly in skeletal muscle, heart, kidney, and placenta. The predicte
d product, a 627-amino-acid protein, exhibited significant sequence homolog
y to the canine 68-kd subunit of the signal recognition particle that has b
een implicated in the transport of secreted and membrane proteins to the en
doplasmic reticulum for proper processing. We confirmed the location of thi
s gene at chromosome 17q25.1 by radiation-hybrid mapping and by fluorescenc
e in situ hybridization.