Molecular cloning, tissue expression, and chromosomal assignment of a novel gene encoding a subunit of the human signal-recognition particle

Citation
H. Harada et al., Molecular cloning, tissue expression, and chromosomal assignment of a novel gene encoding a subunit of the human signal-recognition particle, J HUM GENET, 46(2), 2001, pp. 70-75
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
JOURNAL OF HUMAN GENETICS
ISSN journal
1434-5161 → ACNP
Volume
46
Issue
2
Year of publication
2001
Pages
70 - 75
Database
ISI
SICI code
1434-5161(2001)46:2<70:MCTEAC>2.0.ZU;2-H
Abstract
Human cancers derived from breast, esophageal, or ovarian tissues frequentl y show allelic losses on chromosome band 17q25. Moreover, a locus responsib le for hereditary focal nonepidermolytic palmoplantar keratoderma, a condit ion associated with esophageal cancer (TOC; tylosis with oesophageal cancer ), has been mapped to the same band. During efforts to sequence, by shotgun methods, a 1-Mb target region that we had defined as the DNA segment harbo ring the putative tumor suppressor gene(s) involved in these events, we ide ntified a novel cDNA. The full-length cDNA is 2495bp long and is expressed predominantly in skeletal muscle, heart, kidney, and placenta. The predicte d product, a 627-amino-acid protein, exhibited significant sequence homolog y to the canine 68-kd subunit of the signal recognition particle that has b een implicated in the transport of secreted and membrane proteins to the en doplasmic reticulum for proper processing. We confirmed the location of thi s gene at chromosome 17q25.1 by radiation-hybrid mapping and by fluorescenc e in situ hybridization.