Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR

Citation
A. Miyamoto et al., Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR, INT J ONCOL, 18(3), 2001, pp. 527-532
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN journal
1019-6439 → ACNP
Volume
18
Issue
3
Year of publication
2001
Pages
527 - 532
Database
ISI
SICI code
1019-6439(200103)18:3<527:CAOQAF>2.0.ZU;2-A
Abstract
In order to detect a hematogenous spread of tumor cells in patients with he patocellular carcinoma, reverse transcription-polymerase chain reaction ass ay has been used. In this study, we quantified a-fetoprotein (AFP) messenge r RNA by real-time PCR approach using LightCycler(TM) technique. AFP messen ger RNA in the blood from 23 hepatocellular carcinoma patients undergoing h epatic resection, 31 healthy volunteers, 10 patients with liver cirrhosis a nd 5 patients underwent hepatectomy except for hepatocellular carcinoma was quantitated. In the real-time PCR, fluorescence was undetectable in any of the controls. On the contrary, fluorescent signals were detected in 10 out of 39 blood specimens collected from 23 HCC patients. AFP-positive status was significantly associated with the existence of multiple intrahepatic no dules. Out of 8 cases with AFP-positive status, intra- and/or extra-hepatic recurrence has been observed in 3 cases. The quantities of AFP messenger R NA in these 3 cases were relatively high among 8 cases with AFP-positive st atus. AFP messenger RNA was detectable by newly developed real-time PCR app roach with LightCycler and it is suggested that this approach could be appl icable in detection of small amounts of tumor cells in the blood of HCC pat ients.