Characterization of low dose streptozotocin-induced progressive diabetes in mice

Citation
M. Ito et al., Characterization of low dose streptozotocin-induced progressive diabetes in mice, ENV TOX PH, 9(3), 2001, pp. 71-78
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
ISSN journal
1382-6689 → ACNP
Volume
9
Issue
3
Year of publication
2001
Pages
71 - 78
Database
ISI
SICI code
1382-6689(200101)9:3<71:COLDSP>2.0.ZU;2-0
Abstract
Our previous study indicated that a single i.p. injection of 100 mg/kg stre ptozotocin (STZ) is able to induce slowly progressive diabetes mellitus in adult ICR mice. In the present study, the non-fasting serum insulin levels of the mice administered 100 mg/kg STZ were normal throughout the 24-week-o bservation after STZ injection. In the STZ-administered mice, the area of i slets and the number of insulin-immunoreactive cells (beta -cells) were nor mal at I week and then continued to decrease gradually as the time went on. In contrast, there was a relative increase in the number of glucagon-immun oreactive cells (alpha -cells) in these mice. In addition, in the STZ-admin istered mice, the degree of glucose tolerance continued to reduce from 2 we eks till 12 weeks when the experiment terminated. The rise in serum insulin levels stimulated by glucose in the STZ-administered mice began to subside from about 2 weeks and had completely ceased by 12 weeks. These results in dicate that 100 mg/kg STZ-induced diabetic mouse model is non-insulin-depen dent diabetes, which is characterized by impaired insulin response to gluco se stimulation. (C) 2001 Elsevier Science B.V. All rights reserved.