Intestinal uptake of betaine in vitro and the distribution of methyl groups from betaine, choline, and methionine in the body of broiler chicks

Citation
H. Kettunen et al., Intestinal uptake of betaine in vitro and the distribution of methyl groups from betaine, choline, and methionine in the body of broiler chicks, COMP BIOC A, 128(2), 2001, pp. 269-278
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Animal Sciences",Physiology
Journal title
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
ISSN journal
1095-6433 → ACNP
Volume
128
Issue
2
Year of publication
2001
Pages
269 - 278
Database
ISI
SICI code
1095-6433(200102)128:2<269:IUOBIV>2.0.ZU;2-4
Abstract
The efficiency of betaine absorption into small intestinal slices of broile r chicks was studied in vitro with C-14-labeled betaine. The relative propo rtion of Naf-coupled betaine uptake, as well as the total uptake capacity w as larger in the duodenum than in the jejunum. Dietary betaine increased th e Na+-coupled uptake in the duodenum. In in vivo-experiments, methyl-C-14-l abeled betaine, methionine, or choline was fed to broiler chicks. Betaine a ppeared in the blood more rapidly, and reached a higher total concentration than choline or methionine. The data suggest that choline and methionine w ere associated with plasma lipoproteins whereas betaine remained fret: in t he plasma. The label distribution in liver, kidney, and intestinal tissues was studied 24 h after label ingestion. Most of the label from betaine was found in the aquaeous phase in the muscle, while in the liver and jejunum t he label from betaine was distributed more evenly between the aquaeous, lip id, and protein phases. Label from choline accumulated in the lipid fractio n, particularly so in the liver, whereas label from methionine showed a mor e variable distribution pattern. The distribution results are interpreted i n terms of specific roles of betaine, choline, and methionine in methyl gro up metabolism. (C) 2001 Elsevier Science Inc. All rights reserved.