Reference interval and subject variation in excretion of urinary metabolites of nicotine from non-smoking healthy subjects in Denmark

Am. Hansen et al., Reference interval and subject variation in excretion of urinary metabolites of nicotine from non-smoking healthy subjects in Denmark, CLIN CHIM A, 304(1-2), 2001, pp. 125-132
Citations number
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
ISSN journal
0009-8981 → ACNP
Year of publication
125 - 132
SICI code
Background: Passive smoking has been found to be a respiratory health hazar d in humans. The present study describes the calculation of a reference int erval for urinary nicotine metabolites calculated as cotinine equivalents o n the basis of 77 non-smokers exposed to tobacco smoke less than 25% of the day. Methods: Twenty subjects (passive smokers) exposed to tobacco smoke m ore: than 25% of the day (subjectively assessed) and 32 smokers were used t o validate the estimated reference interval. Urine samples were collected t hree times during the day approximately at 06.30. 17.00 and 22.45 h. Result s: Within-subject variation was found to be 89.4, 72.6. and 79.2% and betwe en-subject variation was found to be 64.5. 64.2, and 36.1%. No gender diffe rence could be demonstrated. In general all subjects showed increased conce ntrations in the afternoon and evening samples compared to the morning samp les. Parametric reference interval fur excretion of nicotine metabolites in urine from non-smokers was established according to International Union of Pure and Applied Chemistry (IUPAC) and International Federation Tor Clinic al Chemistry (IFCC) For use of risk assessment of exposure to tobacco smoke . The reference interval For urinary cotinine was estimated to be 1.1-90.0 mu mol/mol creatinine in morning samples From non-smokers. An intercomparis on between the radioimmunoassay (RIA, method used for determination of nico tine metabolites and a pas chromatography-mass spectrometry (GC-MSI method for determination of cotinine was carried out on 27 samples from non-smoker s and smokers. Results obtained from the RIA method showed 2.84 [confidence interval (CI): 2.50; 3.18] times higher results compared to the GC-MS meth od. A linear correlation between the two methods was demonstrated (rho = 0. 96). Conclusion: The RIA method is rapid and adequate for clinical use in t he assessment of exposure to tobacco smoke. i.e. ratio between CVa/CVt, was <0.50. (C) 2001 Elsevier Science B.V. All rights reserved.