Dorsal horn lesion resulting from spinal root avulsion leads to the accumulation of stress-responsive proteins

Citation
H. Nomura et al., Dorsal horn lesion resulting from spinal root avulsion leads to the accumulation of stress-responsive proteins, BRAIN RES, 893(1-2), 2001, pp. 84-94
Citations number
60
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
BRAIN RESEARCH
ISSN journal
0006-8993 → ACNP
Volume
893
Issue
1-2
Year of publication
2001
Pages
84 - 94
Database
ISI
SICI code
0006-8993(20010302)893:1-2<84:DHLRFS>2.0.ZU;2-O
Abstract
The aim of this study was to demonstrate acute to subacute molecular episod es in the dorsal horn following root avulsion using immunohistochemical met hods with the markers for synapses, astrocytes and such stress-responsive m olecules as heat shock proteins (Hsps) and p38 MAP kinase (p38). Among them , Hsp27 was accumulated selectively in the injured substantia gelatinosa 24 h after avulsion injury. The localization of Hsp27 in astrocytes within th e substantia gelatinosa was confirmed by the double immunofluorescence meth od using anti-Hsp27 antibody and either anti-synaptophysin antibody or anti -glutamine synthetase antibody and by immunoelectron microscopy for Hsp27. The pattern of Hsp27 expression subsequently changed from glial pattern to punctate pattern by 7 days. Immunoelectron microscopy revealed that the pun ctate pattern in the subacute stage corresponded to distal parts of the ast rocytic processes. Hsp27 immunoreaction was decreased 21 days after root av ulsion. In the distal axotomy model, Hsp27 was accumulated later in the ips ilateral dorsal horn in a punctate pattern from 7 days after the axotomy. P hosphorylation of p38 was detected in microglia in the dorsal horn followin g both avulsion and axotomy. Substance P was slightly decreased in the inju red substantia gelatinosa in both the avulsion and axotomy models around 14 -21 days. We conclude that Hsp27 is a useful marker for demonstrating dorsa l horn lesions following avulsion injury and that avulsion injury may induc e Hsp27 in the dorsal horn more rapidly then distal axotomy. (C) 2001 Elsev ier Science B.V. All rights reserved.