Small-scale bioreactor system for process development and optimization

Citation
P. Girard et al., Small-scale bioreactor system for process development and optimization, BIOCH ENG J, 7(2), 2001, pp. 117-119
Citations number
3
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOCHEMICAL ENGINEERING JOURNAL
ISSN journal
1369-703X → ACNP
Volume
7
Issue
2
Year of publication
2001
Pages
117 - 119
Database
ISI
SICI code
1369-703X(200103)7:2<117:SBSFPD>2.0.ZU;2-U
Abstract
An agitated 12-well microtiter place system with a working volume of 2 ml w as investigated for cell culture process development. Agitation assures hom ogeneity in wells and enhances mass transfer between the gas and the liquid phase, thus improving maximum cell density and pH stability. The pH of the NaHCO3-buffered system can be adjusted by altering the carbon dioxide cont ent of the gas phase. The non-toxic, visual pH indicator phenol red was use d in combination with a spectrophotometric plate reader for rapid and preci se pH measurements. For high throughputs, cell growth was assessed non-inva sively using stable green fluorescent protein (GFP) expressing cells and a fluorescence plate reader. The setup is simple and inexpensive. The system can be automated and allows several hundred small-scale bioreactor experime nts to be run in parallel. (C) 2001 Elsevier Science B.V. All rights reserv ed.