Pho85 kinase, a yeast cyclin-dependent kinase, regulates the expression ofUGP1 encoding UDP-glucose pyrophosphorylase

Citation
M. Nishizawa et al., Pho85 kinase, a yeast cyclin-dependent kinase, regulates the expression ofUGP1 encoding UDP-glucose pyrophosphorylase, YEAST, 18(3), 2001, pp. 239-249
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
YEAST
ISSN journal
0749-503X → ACNP
Volume
18
Issue
3
Year of publication
2001
Pages
239 - 249
Database
ISI
SICI code
0749-503X(200102)18:3<239:PKAYCK>2.0.ZU;2-C
Abstract
The PHO85 gene is a negative regulator of the PHO system in the S-east Sacc haromyces cerevisiae and encodes a protein kinase (Pho85) highly homologous to the Cdc28 kinase (Cdc28), Ten cyclin-like proteins are known to interac t with Pho85, and combination with different cyclins is believed to be resp onsible for distinct Pho85 functions, including phosphate metabolism, carbo n source utilization and cell cycle regulation. However, only a limited num ber of substrates of Pho85 kinase, including Pho4, Gsy2 and Sicl, have so f ar been identified. To search for more targets of Pho85 and to clarify the genetic control mechanisms by Pho85 kinase in these cellular functions, we carried out a genome-wide analysis of the effect of a pho85 Delta mutation on gene expression. We found that expression of various genes involved in c arbon metabolism are affected by the mutation and that among them, UGP1 pro moter activity was increased in the absence of Pho85 kinase. This increase in the promoter activity. was not observed in a pho4 Delta mutant or with a mutant UGP1 promoter that is devoid of putative Pho4 and Bas2 binding site s, suggesting that UGP1 expression is modulated by Pho85 through Pho4. We a lso found that expression of several Pho85-cyclin genes were altered by the carbon source, the growth phase and Pho85 kinase itself. Copyright (C) 200 0 John Wiley & Sons, Ltd.