Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers

Citation
E. Forgacs et al., Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers, ONCOGENE, 20(8), 2001, pp. 1005-1009
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ONCOGENE
ISSN journal
0950-9232 → ACNP
Volume
20
Issue
8
Year of publication
2001
Pages
1005 - 1009
Database
ISI
SICI code
0950-9232(20010222)20:8<1005:SFMMIC>2.0.ZU;2-8
Abstract
RepX represents a new informatics approach to probe the UniGene database fo r potentially polymorphic repeat sequences in the open reading frame (ORF) of genes, 56% of which were found to be actually polymorphic. We now have p erformed mutational analysis of 17 such sites in genes not found to be poly morphic (<0.03 frequency) in a large panel of human cancer genomic DNAs der ived from 31 lung, 21 breast, seven ovarian, 21 (13 microsatellite instabil ity (MSI)+ and eight MS-) colorectal cancer cell lines. In the lung, breast and ovarian tumor DNAs we found no mutations (<0.03-0.04 rate of tumor ass ociated open reading frame mutations) in these sequences, BS contrast, 18 M SI+ colorectal cancers (13 cancer cell lines and five primary tumors) with mismatch repair defects exhibited six mutations in three of the 17 genes (S REBP-2, TAN-1, GR6) (P<0.000003 compared to all other cancers tested). We c onclude that coding region microsatellite alterations are rare in lung, bre ast, ovarian carcinomas and MSI(-) colorectal cancers, but are relatively f requent in MSI (+) colorectal cancers with mismatch repair deficits.