Control of enzymic activity by artificial cell-surface receptors

Citation
J. Kikuchi et al., Control of enzymic activity by artificial cell-surface receptors, J MOL CAT B, 11(4-6), 2001, pp. 977-984
Citations number
46
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN journal
1381-1177 → ACNP
Volume
11
Issue
4-6
Year of publication
2001
Pages
977 - 984
Database
ISI
SICI code
1381-1177(20010122)11:4-6<977:COEABA>2.0.ZU;2-1
Abstract
A novel type of artificial cell-surface receptor capable of affecting enzym ic activity was designed by employing steroidal moiety. Bile acid derivativ es having an amino group, effectively recognized both the aromatic aldehyde s and copper(II) ions in bilayer membrane formed with a synthetic peptide l ipid. On the other hand, pig heart L-lactate dehydrogenase (LDH) was bound to the bilayer membrane surface mainly through electrostatic interactions, and the LDH activity was specifically inhibited by copper(LI) ions. On thes e grounds, we constituted artificial signaling systems in combinations, wit h a steroidal receptor, a bilayer-forming synthetic lipid, and LDH. The pre sent supramolecular vesicles acted as nano-reactors in which the artificial receptor was able to switch on the enzymic activity via accompanying doubl e signal recognition and phase reorganization (C) 2001 Elsevier Science B.V . All rights reserved.