Improving enzyme characteristics by gene shuffling; application to beta-glucosidase

Citation
K. Hayashi et al., Improving enzyme characteristics by gene shuffling; application to beta-glucosidase, J MOL CAT B, 11(4-6), 2001, pp. 811-816
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
ISSN journal
1381-1177 → ACNP
Volume
11
Issue
4-6
Year of publication
2001
Pages
811 - 816
Database
ISI
SICI code
1381-1177(20010122)11:4-6<811:IECBGS>2.0.ZU;2-R
Abstract
The genes of family 3 beta -glucosidase enzymes consist of five distinct re gions; the N-terminal residues, an N-terminal catalytic domain, a nonhomolo gous region, a C-terminal domain of unknown function and the C-terminal res idues. The beta -glucosidase genes derived from Cellvibrio gilvus (CG) and Agrobacterium tumefaciens (AT) have been subjected to gene deletion, trunca tion and shuffling. The folding information was found to be distributed une venly across the different regions based on the gene manipulation results. Chimeric enzymes with improved enzyme characteristics were obtained only by gene shuffling at the C-terminal domain. (C) 2001 Elsevier Science B.V. Al l rights reserved.