Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides Bunge

Citation
Y. Takeda et al., Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides Bunge, J GASTRO, 36(2), 2001, pp. 79-90
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology
Journal title
JOURNAL OF GASTROENTEROLOGY
ISSN journal
0944-1174 → ACNP
Volume
36
Issue
2
Year of publication
2001
Pages
79 - 90
Database
ISI
SICI code
0944-1174(200102)36:2<79:GIAAOG>2.0.ZU;2-Q
Abstract
In this study, we aimed to determine the growth inhibition and the inductio n of apoptotic cell death brought about by the herb Anemarrhena asphodeloid es Bunge in gastric cancer cell lines, and to clarify the mechanism of this apoptosis. Water-soluble ingredients of A. asphodeloides, and the gastric cancer cell lines, MKN45 and KATO-III, were used in vitro. Growth inhibitio n, induction of cell death, morphological features, the presence of DNA lad ders, increases in caspase-3-like activity, the effects of a caspase-3 inhi bitor on apoptotic cell death, and the release of cytochrome c by A. asphod eloides were analyzed. A. asphodeloides inhibited the growth and decreased the viability of the gastric cancer cell lines. The viability of normal ski n fibroblasts in the presence of low concentrations of A. asphodeloides was higher than that of gastric cancer cells. Apoptotic bodies and DNA ladders were observed to be induced in MKN45 and KATO-III by A. asphodeloides. The caspase 3 inhibitor, Ac-DEVD-CHO, inhibited the apoptotic cell death of ga stric cancer cells induced by A. asphodeloides. The caspase 3-like activity in MKN45 and KATO-III cells increased after the addition of A. asphodeloid es. Cytochrome c was released from mitochondria into the cytosol 8 h after the addition of A. asphodeloides, and reached a peak at 16 h. The peak of c ytochrome c release was earlier than that of caspase 3-like activity. We co ncluded that A. asphodeloides inhibited the growth of the gastric cancer ce ll lines MKN45 and KATO-III and induced apoptosis. The apoptosis of MKN45 a nd KATO-III cells induced by A. asphodeloides was associated with the relea se of cytochrome c from the mitochondria, followed by an increase in caspas e 3-like activity.