Molecular characterization and diversity of thermophilic iron-reducing enrichment cultures from deep subsurface environments

Citation
J. Zhou et al., Molecular characterization and diversity of thermophilic iron-reducing enrichment cultures from deep subsurface environments, J APPL MICR, 90(1), 2001, pp. 96-105
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF APPLIED MICROBIOLOGY
ISSN journal
1364-5072 → ACNP
Volume
90
Issue
1
Year of publication
2001
Pages
96 - 105
Database
ISI
SICI code
1364-5072(200101)90:1<96:MCADOT>2.0.ZU;2-5
Abstract
Aims: The objectives of this work were to explore the diversity in Fe(III)- reducing enrichment cultures from the deep subsurface and to identify; stra ins involved in metal reduction. Methods and Results: Analyses of 16S ribosomal RNA (rRNA) of enrichments, s upplemented with hydrogen, acetate or pyruvate as an electron donor, identi fied three dominant operational taxonomic units (OTUs). All cultures exhibi ted considerable diversity (36-24 OTUs), even after being transferred at le ast nine times. Two OTUs were present in all three cultures, constituting a bout 65% of the total clones examined. Conclusions: Dominant OTUs appeared to be most closely related to Thermoana erobacter ethanolicus or T. kivui. One OTU, which is potentially responsibl e for autotrophic Fe(III) reduction, was only about 95% similar to T. ethan olicus and may represent a new species. Significance and Impact of the Study: An unexpectedly high diversity was fo und in these enrichments and this diversity may be a feature that can be ex ploited.