Disialoganglioside GD3 is selectively expressed by developing and mature human mast cells

Citation
Sl. Ren et al., Disialoganglioside GD3 is selectively expressed by developing and mature human mast cells, J ALLERG CL, 107(2), 2001, pp. 322-330
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
ISSN journal
0091-6749 → ACNP
Volume
107
Issue
2
Year of publication
2001
Pages
322 - 330
Database
ISI
SICI code
0091-6749(200102)107:2<322:DGISEB>2.0.ZU;2-0
Abstract
Background: Disialoganggioside GD3 is expressed on the surface of selected cell types, Anti-GD3 mAb administered to human subjects with malignant mela noma produces signs and symptoms of immediate hypersensitivity reactions. Objective: The expression of GD3 by human mast cells was assessed during ma st cell development in vitro and in samples of lung and skin. Methods: GD3 on tissue- and in vitro-derived mast cells was analyzed after double labeling of cells for tryptase (G3 mAb) or Kit (YB5.B8 mAb) and GD3 (R24 mAb). Glycolipids in extracts of fetal liver-derived mast cells were e xamined by using high-performance thin-layer chromatography. Results: Flow cytometry showed that the percentage of GD3(+) cells increase d in parallel to Kit(+) cells during the recombinant human stem cell factor -dependent development of fetal liver-derived mast cells. Double-labeling e xperiments showed that GD3(+) cells were also surface Kit(+) and granule tr yptase positive, identifying them as mast cells in preparations of lung-, s kin-, fetal liver-, and cord blood-derived cells. The major acidic glycolip id detected was NeuAc alpha2-8NeuAc alpha2-3Gal beta1-4Glc beta1-1'Cer (GD3 ). Among peripheral blood leukocytes, only basophils and about 10% of the T cells were labeled with anti-GD3 mAb. Anti-GD3 mAb-conjugated magnetic bea ds were used to purify mast cells to greater than 90% purity from dispersed skin cells enriched to approximately 12% purity by means of density-depend ent sedimentation but were less proficient for dispersed human lung mast ce lls, most likely because of other cell types that express GD3. Conclusion: GD3 is expressed on the surface of developing human mast cells in parallel to tryptase in secretory granules and, like Kit, can serve as a target for their enrichment by immunoaffinity techniques.