Isolation of lectin and albumin from Pisum sativum var. macrocarpon ser. cv. sugar snap

Authors
Citation
Xy. Ye et Tb. Ng, Isolation of lectin and albumin from Pisum sativum var. macrocarpon ser. cv. sugar snap, INT J BIO C, 33(1), 2001, pp. 95-102
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
ISSN journal
1357-2725 → ACNP
Volume
33
Issue
1
Year of publication
2001
Pages
95 - 102
Database
ISI
SICI code
1357-2725(200101)33:1<95:IOLAAF>2.0.ZU;2-3
Abstract
A mannose- and glucose-binding lectin bearing considerable sequence similar ity to other legume lectins was isolated using a simple procedure, from leg umes of the sugar snap Pisum sativum var, macrocarpon. The lectin was unads orbed on Affi-gel blue gel and Q-Sepharose in 10 mM Tris-HCl buffer (pH 7.2 ) and adsorbed on SP-Toyopearl in 50 mM NaOAc buffer (pH 5). An albumin cou ld also be purified at the same time. It was unadsorbed on Affi-gel Blue ge l, adsorbed on Q-Sepharose and unadsorbed on SP-Toyopearl under the aforeme ntioned chromatographic conditions. The lectin was almost identical in N-te rminal sequences of its alpha- and beta -subunit to lectin from P. sativum L. var. Feltham First except for the 19th N-terminal residue of the beta -s ubunit. The lectin was devoid of antifungal activity. Out of the 15 N-termi nal amino acids examined in pea albumin, three were different between the t wo varieties of P. sativum. (C) 2001 Elsevier Science Ltd. All rights reser ved.