Recognition of the Lck tyrosine kinase as a tumor antigen by cytotoxic T lymphocytes of cancer patients with distant metastases

Citation
N. Harashima et al., Recognition of the Lck tyrosine kinase as a tumor antigen by cytotoxic T lymphocytes of cancer patients with distant metastases, EUR J IMMUN, 31(2), 2001, pp. 323-332
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
ISSN journal
0014-2980 → ACNP
Volume
31
Issue
2
Year of publication
2001
Pages
323 - 332
Database
ISI
SICI code
0014-2980(200102)31:2<323:ROTLTK>2.0.ZU;2-V
Abstract
The Lck protein (p56(lck)), a src family tyrosine kinase that is essential for T cell development and function, is aberrantly expressed in metastatic colon cancers. p56(lck) seems to facilitate the malignant transformation of epithelial cells through initiation of anchorage-independent proliferation . We demonstrate that the lck gene encodes antigenic epitopes recognized by the HLA class I-restricted and tumor-specific CTL of metastatic cancer pat ients. Lck peptides augmented CTL activity in peripheral blood mononuclear cells (PBMC) of colon and other epithelial cancer patients with distant met astases, but not those without distant metastases. CTL precursors recognizi ng the Lck peptide were identified in freshly prepared PBMC of patients wit h distant metastases, and their frequency was significantly augmented by st imulation with the peptide. Thus, Lck peptides could be useful in developin g a specific immunotherapy for cancer patients with distant metastases.