Optimal induction of effector but not memory antitumor cytotoxic T lymphocytes involves direct antigen presentation by the tumor cells

Citation
Y. Guilloux et al., Optimal induction of effector but not memory antitumor cytotoxic T lymphocytes involves direct antigen presentation by the tumor cells, CANCER RES, 61(3), 2001, pp. 1107-1112
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER RESEARCH
ISSN journal
0008-5472 → ACNP
Volume
61
Issue
3
Year of publication
2001
Pages
1107 - 1112
Database
ISI
SICI code
0008-5472(20010201)61:3<1107:OIOEBN>2.0.ZU;2-L
Abstract
MHC class I-restricted tumor antigen can be presented to CD8(+) T cells by two distinct mechanisms. Direct presentation involves degradation of cytoso lic proteins by the proteosome into peptides, transport of the peptides acr oss the endoplasmic reticulum membrane, and expression of the MHC-peptide c omplex on the tumor cell surface. Cross-presentation, on the other hand, in volves uptake and intracellular processing of the tumor antigen by host ant igen-presenting cells. Whereas it is dear that cross-presentation is necess ary and sufficient for the induction of memory CTLs, it has not been tested whether such presentation is sufficient to induce effector CTLs, Here we a nalyzed the requirements of direct antigen presentation for the induction o f effector and memory antitumor CTLs using a MHC class I- mutant incapable of direct antigen presentation and its parent, the MHC class I+ J558 cell l ine. We report that in comparison with the MHC class I+ tumor cell, the MHC class I- mutant induces equal priming for recall CTL response but poor eff ector CTLs, Our results demonstrate that optimal induction of effector CTLs , but not memory CTLs, requires direct antigen presentation by the tumor ce lls.