Comparative efficacy as antioxidants between ascorbic acid and epigallocatechin gallate on cells of two human lymphoblastoid lines

Citation
Tc. Hsu et al., Comparative efficacy as antioxidants between ascorbic acid and epigallocatechin gallate on cells of two human lymphoblastoid lines, CANC GENET, 124(2), 2001, pp. 169-171
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER GENETICS AND CYTOGENETICS
ISSN journal
0165-4608 → ACNP
Volume
124
Issue
2
Year of publication
2001
Pages
169 - 171
Database
ISI
SICI code
0165-4608(20010115)124:2<169:CEAABA>2.0.ZU;2-R
Abstract
Both ascorbic acid and epigallocatechin gallate, which is one of the key po lyphenols contained in green tea leaves, have been considered excellent ant ioxidants. The present study compared the efficacy as antioxidants between the two agents on an equimolar basis. Cells of two lymphoid Lines were used as test material to determine the reduction of chromosome damage induced b y the radiomimetic antibiotic bleomycin. Without bleomycin, both agents, at concentrations of 10(-7), 10(-6), 10(-5), and 10(-4) M, showed chromosome damage similar to the untreated controls. With bleomycin, the weakest conce ntration of both showed no protective effect. At concentrations of 10(-6) a nd 10(-5) M, especially the latter, a significant reduction in frequencies of chromatid breaks was recorded. However, at the highest concentration, 10 (-4) M, the chromatid break frequencies rose to the same level as that of c ells treated with bleomycin alone: suggesting that both behaved like prooxi dants. (C) 2001 Elsevier Science Inc. All rights reserved.