Fine-needle aspiration cytology of Hodgkin disease - A study of 89 cases with emphasis on the false-negative cases

Citation
Dc. Chhieng et al., Fine-needle aspiration cytology of Hodgkin disease - A study of 89 cases with emphasis on the false-negative cases, CANC CYTOP, 93(1), 2001, pp. 52-59
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER CYTOPATHOLOGY
ISSN journal
0008-543X → ACNP
Volume
93
Issue
1
Year of publication
2001
Pages
52 - 59
Database
ISI
SICI code
0008-543X(20010225)93:1<52:FACOHD>2.0.ZU;2-Q
Abstract
INTRODUCTION. Although the cytologic features of Hodgkin disease (HD) has b een well described, HD accounts for most of the false-negative fine-needle aspiration (FNA) biopsies of malignant lymphomas. In this study, the author s examined the factors contributing to a false-negative diagnosis of HD. METHODS. Eighty-nine cases from 72 patients (23 females and 49 males) with HD evaluated by FNA were identified between 1990 and 1999. The patients' ag es ranged from 5 to 90 years (median, 38 years). Eighty-five FNAs were from lymph nodes, and 4 were from extranodal sites. Histologic correlation was available for all patients. RESULTS. Based on the original cytologic diagnosis, 43 (48.3%) cases had a positive diagnosis of HD, 20 (22.5%) suspicious or atypical diagnosis, 13 ( 14.6%) a benign diagnosis (false-negative cases), and 10 (11.2%) were nondi agnostic. Three (3.4%) additional cases had a malignant diagnosis other tha n HD. After review, three false-negative cases were reclassified as HD and seven as atypical lymphoid proliferation. Three of these 10 cases also show ed conspicuous collections of histiocytes mimicking poorly formed granuloma s. In those "atypical" cases, only rare Reed-Sternberg (R-S) cells variants were identified. No R-S cells or its variants were identified in the remai ning three false-negative cases; subsequent excisional biopsy showed partia l involvement of the lymph node by HD in two cases. Among the nondiagnostic cases, nine cases showed considerable fibrosis in the resected lymph node. In addition, six cases were performed without on-site assessment. CONCLUSIONS. The cytologic diagnosis of HD can be challenging when classic R-S cells are absent. Contributing factors for a false-negative diagnosis i nclude obscuring reactive inflammatory cells, fibrosis of the involved lymp h nodes, partial involvement of the lymph node by HD, sampling error, and m isinterpretation. On-site assessment significantly minimizes the false-nega tive diagnostic rate. Furthermore, additional material can be obtained for ancillary studies. Cancer (Cancer Cytopathol) 2001;93:52-59. (C) 2001 Ameri can Cancer Society.