Correlation between microsatellite instability and metachronous disease recurrence after endoscopic mucosal resection in patients with early stage gastric carcinoma

Citation
A. Kawamura et al., Correlation between microsatellite instability and metachronous disease recurrence after endoscopic mucosal resection in patients with early stage gastric carcinoma, CANCER, 91(2), 2001, pp. 339-345
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Oncology,"Onconogenesis & Cancer Research
Journal title
CANCER
ISSN journal
0008-543X → ACNP
Volume
91
Issue
2
Year of publication
2001
Pages
339 - 345
Database
ISI
SICI code
0008-543X(20010115)91:2<339:CBMIAM>2.0.ZU;2-4
Abstract
BACKGROUND. Since endoscopic treatment was first evaluated and established as a treatment for patients with early stage gastric carcinoma, metachronou s disease recurrence at other sites in the stomach after endoscopic treatme nt has become a major problem. METHODS. A retrospective case-control study was conducted on 10 patients wi th metachronous recurrence of gastric carcinoma after undergoing successful endoscopic mucosal resection (EMR) therapy for early stage gastric carcino ma and on 14 patients without recurrence. Gastric mucosal tissues obtained during the initial EMR were dissected, and DNA samples from the tumor tissu e and surrounding nonneoplastic mucosa were extracted separately, Microsate llite instability (MSI) was tested in five microsatellite markers (D2S137, D3S1067, TP53, TGF beta RII, and BAX) The authors also looked for K-ras cod on 12 point mutations in the tumor tissues. In addition, immunohistochemica l staining was done to test for the presence of proliferating cell nuclear antigen (PCNA), p53, hMSH2, and hMLH1 in the mucosal tissues. Finally, the correlation between the presence or absence of metachronous recurrence and the characteristics of the primary tumor (MSI, K-ras, p53, etc.) were inves tigated. RESULTS, Three of 10 patients with recurrent disease showed MSI in more tha n two microsatellite markers among 3-5 investigated site (MSI-H), whereas n one of the patients with nonrecurrent disease did so. There was no signific ant correlation between metachronous recurrence after EMR and immunohistoch emical staining reactions, including those for PCNA, p53, hMSH2, and hMLH1. None of the patients showed K-rns mutations. CONCLUSIONS, Thirty percent of patients with recurrent disease showed MSI-H , whereas none of the patients with nonrecurrent disease did so. (C) 2001 A merican Cancer Society.