Coulometric titration of D(+)-glucose using its enzymatic oxidation

Citation
T. Tanaka et al., Coulometric titration of D(+)-glucose using its enzymatic oxidation, ANAL SCI, 17(2), 2001, pp. 277-280
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL SCIENCES
ISSN journal
0910-6340 → ACNP
Volume
17
Issue
2
Year of publication
2001
Pages
277 - 280
Database
ISI
SICI code
0910-6340(200102)17:2<277:CTODUI>2.0.ZU;2-1
Abstract
A definitive method is described for the indirect assay of milligram quanti ties of D(+)-glucose by coulometric titration. D(+)-Glucose was aerobically oxidized by glucose oxidase in an acetate buffer solution (pH 5.1). Subseq uently, the enzymatically formed hydrogen peroxide was titrated coulometric ally with electrogenerated hypobromite in sodium bromide-sodium tetraborate medium of pH 8.6, with biamperometric end-point detection. Parameters affe cting the enzymatically catalyzed oxidation acid coulometric titration were evaluated. The optimized conditions for the oxidation of up to 20 mg of D( +)-glucose include the addition of 4500 U of glucose oxidase and stirring o ver a 10-min interval at 25 degreesC. Under proposed conditions, the assay values of several commercial D(+)-glucose reagents were somewhat lower than the guaranteed minimum values, with RSDs (n = 5) of 0.071 - 0.106%.