Palladium film decoupler for amperometric detection in electrophoresis chips

Citation
Dc. Chen et al., Palladium film decoupler for amperometric detection in electrophoresis chips, ANALYT CHEM, 73(4), 2001, pp. 758-762
Citations number
40
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
0003-2700 → ACNP
Volume
73
Issue
4
Year of publication
2001
Pages
758 - 762
Database
ISI
SICI code
0003-2700(20010215)73:4<758:PFDFAD>2.0.ZU;2-3
Abstract
Demonstrated in this article is that a palladium metal film can be applied to decouple the electric circuitry of electrochemical detection from that o f the electrophoretic separation in an electrophoresis chip. The Pd solid-s tate field decoupler, as well as the working electrodes, is thermally evapo rated onto the plastic chip and oriented vertically across the separation c hannel. After the sample zones flow over the Pd decoupler, their electroche mical response is measured at working electrodes in the downstream pathway. Because the electrodes are on the separation channel, the electrode channe l alignment is no longer a problem. For a separation channel of roughly 200 mum in width and 75 mum in depth in 10 mM phosphate (pH 5.1), the noise le vel at the working electrode is < 15 pA at an electric field of 570 V/cm.