Rapid amplified ribosomal DNA restriction analysis (ARDRA) identification of Lactobacillus spp. isolated from fecal and vaginal samples

Citation
M. Ventura et al., Rapid amplified ribosomal DNA restriction analysis (ARDRA) identification of Lactobacillus spp. isolated from fecal and vaginal samples, SYST APPL M, 23(4), 2000, pp. 504-509
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN journal
0723-2020 → ACNP
Volume
23
Issue
4
Year of publication
2000
Pages
504 - 509
Database
ISI
SICI code
0723-2020(200012)23:4<504:RARDRA>2.0.ZU;2-W
Abstract
Amplified ribosomal DNA restriction analysis (ARDRA) was used to identify d ifferent species of Lactobacillus isolated from human faeces and vagina. PC R-ARDRA was performed using a set of four restriction enzymes, able to diff erentiate fourteen species of Lactobacillus. The PCR-ARDRA procedure descri bed in this paper was shown to be a reliable and rapid method for identifyi ng Lactobacillus species from intestinal and vaginal microflora at species and subspecies level.