Genomic fingerprints, ARDRA profiles and quinone systems for classification of Pasteurella sensu stricto

Citation
A. Kainz et al., Genomic fingerprints, ARDRA profiles and quinone systems for classification of Pasteurella sensu stricto, SYST APPL M, 23(4), 2000, pp. 494-503
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
SYSTEMATIC AND APPLIED MICROBIOLOGY
ISSN journal
0723-2020 → ACNP
Volume
23
Issue
4
Year of publication
2000
Pages
494 - 503
Database
ISI
SICI code
0723-2020(200012)23:4<494:GFAPAQ>2.0.ZU;2-Q
Abstract
In order to investigate the relationships between species of the genus Past eurella sensu stricto such as Pasteurella multocida, Pasteurella canis, Pas teurella stomatis, Pasteurella dagmatis, Pasteurella avium, Pasteurella vol antium, Pasteurella gallinarum, Pasteurella species A, Pasteurella species B and "Pasteurella leonis" MCCM 00659 their genomic fingerprints and ARDRA profiles were compared and their quinone systems were analysed. Visual comp arison of band patterns from rep-PCR (ERIC-, REP- and BOX-PCR) and the anal yses of the combined band patterns by UPGMA (unweighted. pair group method with averages) dendrogram derived from the combined fingerprint profiles de monstrated that each strain displays a distinct genomic fingerprint. In mem bers of the same species several similarities in the band patterns were obs erved. Combined ARDRA profiles, obtained after digestion of amplified 23S r RNA coding genes with the enzymes DdeI, MseI and RsaI, revealed a dissectio n of the members of the genus Pasteurella sensu stricto into two groups whi ch was in agreement with the two groups obtained from our analyses of the q uinone systems. These two groups corresponded with the hva phylogenetically determined subclusters 3A and 3B described previously The species of subcl uster 3A displayed a quinone system with ubiquinone Q-7 (32-56%) and ubiqui none Q-8 (44-63%) as major compounds. Members of subcluster 3B had a quinon e system with ubiquinone Q-8 (86-97%) as the major compound. Based on these results it can be suggested that the genus Pasteurella sensu stricto shoul d be restricted to the species of subcluster 3B including the species Paste urella multocida, Pasteurella canis, Pasteurella stomatis, Pasteurella dagm atis and Pasteurella species B. In addition, evidence was found which would indicate that: 1) Pasteurella canis MCCM 00927 is misnamed and should be r eclassified with Pasteurella multicida; 2) Pasteurella multicida subsp. sep tica may be classified its a separate species; and 3) "Pasteurella leonis" MCCM 00659 represents a separate species within subcluster 3B and thus coul d be described as a species of Pasteurella sensu stricto (also in a redefin ed genus) when more strains become available.