A. Kainz et al., Genomic fingerprints, ARDRA profiles and quinone systems for classification of Pasteurella sensu stricto, SYST APPL M, 23(4), 2000, pp. 494-503
In order to investigate the relationships between species of the genus Past
eurella sensu stricto such as Pasteurella multocida, Pasteurella canis, Pas
teurella stomatis, Pasteurella dagmatis, Pasteurella avium, Pasteurella vol
antium, Pasteurella gallinarum, Pasteurella species A, Pasteurella species
B and "Pasteurella leonis" MCCM 00659 their genomic fingerprints and ARDRA
profiles were compared and their quinone systems were analysed. Visual comp
arison of band patterns from rep-PCR (ERIC-, REP- and BOX-PCR) and the anal
yses of the combined band patterns by UPGMA (unweighted. pair group method
with averages) dendrogram derived from the combined fingerprint profiles de
monstrated that each strain displays a distinct genomic fingerprint. In mem
bers of the same species several similarities in the band patterns were obs
erved. Combined ARDRA profiles, obtained after digestion of amplified 23S r
RNA coding genes with the enzymes DdeI, MseI and RsaI, revealed a dissectio
n of the members of the genus Pasteurella sensu stricto into two groups whi
ch was in agreement with the two groups obtained from our analyses of the q
uinone systems. These two groups corresponded with the hva phylogenetically
determined subclusters 3A and 3B described previously The species of subcl
uster 3A displayed a quinone system with ubiquinone Q-7 (32-56%) and ubiqui
none Q-8 (44-63%) as major compounds. Members of subcluster 3B had a quinon
e system with ubiquinone Q-8 (86-97%) as the major compound. Based on these
results it can be suggested that the genus Pasteurella sensu stricto shoul
d be restricted to the species of subcluster 3B including the species Paste
urella multocida, Pasteurella canis, Pasteurella stomatis, Pasteurella dagm
atis and Pasteurella species B. In addition, evidence was found which would
indicate that: 1) Pasteurella canis MCCM 00927 is misnamed and should be r
eclassified with Pasteurella multicida; 2) Pasteurella multicida subsp. sep
tica may be classified its a separate species; and 3) "Pasteurella leonis"
MCCM 00659 represents a separate species within subcluster 3B and thus coul
d be described as a species of Pasteurella sensu stricto (also in a redefin
ed genus) when more strains become available.