Alpha-fetoprotein producing gastric cancer lacks transcription factor ATBF1

Citation
H. Kataoka et al., Alpha-fetoprotein producing gastric cancer lacks transcription factor ATBF1, ONCOGENE, 20(7), 2001, pp. 869-873
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ONCOGENE
ISSN journal
0950-9232 → ACNP
Volume
20
Issue
7
Year of publication
2001
Pages
869 - 873
Database
ISI
SICI code
0950-9232(20010215)20:7<869:APGCLT>2.0.ZU;2-6
Abstract
Alpha-fetoprotein (AFP) producing gastric cancer (AFP-GC) is very malignant and highly metastatic compared with common gastric cancer. However, the ca usal relationship between AFP production and the high malignancy of AFP-GC is unclear. We investigated AFP gene regulation in AFP-GC by an active tran scription factor, HNF1 (hapatocyte nuclear factor 1) and a repressive trans cription factor, ATBF1 (AT motif binding factor 1), RNase protection assays revealed that the production of AFP in gastric cancer cells did not direct ly associate with HNF1 expression. An inverse relation between the expressi ons of ATBF1 and AFP was clearly observed in gastric cancer cells. CAT assa ys showed the direct inhibition of AFP gene expression by ATBF1, Methylatio n analysis of the AFP promoter region in gastric cancer cells suggested tha t methylation itself could not explain the silencing of the AFP gene. Immun ohistochemistry of resected clinical samples revealed that AFP producing ce lls lacked ATBF1 immunoreactivity, Our data suggests that the absence of AT BF1 is responsible for AFP gene expression in gastric cancer, and the absen ce of ATBF1 is a distinct characteristic of AFP-GC and might be important f or its highly malignant nature.