Suppression of pulmonary antibacterial defenses mechanisms and lung damagein mice exposed to fluoride aerosol

Citation
S. Yamamoto et al., Suppression of pulmonary antibacterial defenses mechanisms and lung damagein mice exposed to fluoride aerosol, J TOX E H A, 62(6), 2001, pp. 485-494
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Environment/Ecology,"Pharmacology & Toxicology
Journal title
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A
ISSN journal
1528-7394 → ACNP
Volume
62
Issue
6
Year of publication
2001
Pages
485 - 494
Database
ISI
SICI code
1528-7394(200103)62:6<485:SOPADM>2.0.ZU;2-V
Abstract
In endemic fluorosis areas in China associated with coal burning, indoor ai rborne fluoride pollution is severe. To determine the effects of fluoride a erosols on pulmonary antibacterial defense mechanisms and lung damage, mice were exposed to various concentrations of fluoride aerosol (2, 5, or 10 mg /m(3)) or filtered air (control) for 14 d, 4 h/d in an inhalation chamber. Bactericidal activity against Staphylococcus aureus in the lung and the num ber and profile of free pulmonary cells, protein content, and lactate dehyd rogenase (LDH) activity in bronchoalveolar lavage (BAL) fluid were assessed . Urinary fluoride concentration, an indicator of fluoride exposure, increa sed in proportion to fluoride aerosol concentration in the chamber. Wet lun g weight was significantly higher on d 14 in mice exposed to 10 mg/m(3) tha n in controls. Pulmonary bactericidal activity against S. aureus was concen tration-dependently suppressed at 5 and 10 mg/m(3) fluoride. The number of alveolar macrophages (AMs) in the BAL fluid of the mice not bacterially cha llenged decreased significantly at 10 mg/m(3) fluoride. The number of polym orphonuclear leukocytes and lymphocytes increased significantly at 10 mg/m( 3) fluoride exposure. The concentration of total protein (TP) and albumin i n BAL supernatant increased significantly at 5 and 10 mg/m(3) fluoride expo sure, and LDH activity rose markedly at the higher fluoride concentration. Data indicate that fluoride inhalation produces pulmonary cellular alterati ons that are associated with a diminished ability to cope with infectious b acteria.