Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development

Citation
P. Zheng et al., Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development, HUM REPR, 16(2), 2001, pp. 300-305
Citations number
39
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Reproductive Medicine","da verificare
Journal title
HUMAN REPRODUCTION
ISSN journal
0268-1161 → ACNP
Volume
16
Issue
2
Year of publication
2001
Pages
300 - 305
Database
ISI
SICI code
0268-1161(200102)16:2<300:MORMOI>2.0.ZU;2-C
Abstract
This study compared success of in-vitro maturation of rhesus monkey oocytes in protein-free versus serum-containing culture systems, assessed by embry o development subsequent to IVF. Four media were tested: (i) modified Conna ught Medical Research Laboratories medium (mCMRL-1066); (ii) hamster embryo culture medium-10 (HECM-10); (iii) control: mCMRL-1066 + 20% bovine calf s erum (BCS); (iv) HECM-10 + 20% BCS, Immature oocytes from FSH-stimulated rh esus monkeys were allocated among the media containing ovine FSH (5 mug/ml) and LH (10 mug/ml) and cultured for 36-40 h. Metaphase II ova were insemin ated and putative zygotes were cultured in mCMRL-1066 + 20% BCS until devel opment arrested. Ova matured in all four media had similar (P > 0.05) poten tial to initiate (66, 67, 82 and 69% respectively) and complete meiotic mat uration (60, 50, 76 and 57% respectively). Inseminated ova in all groups ha d similar potential to be fertilized (86, 83, 84 and 90% respectively), cle ave (71, 83, 76 and 90% respectively) and develop to the blastocyst stage ( 19, 17, 16 and 30% respectively). These results indicate for the first time that primate oocytes can be successfully matured in protein-free medium, w ith subsequent blastocyst development, comparable to responses in complex m edium with serum. This finding will facilitate studies on mechanisms regula ting primate oocyte maturation.