PURPOSE: We examined the levels of autoantibodies against prethrombin-1 and
fragment-1 in pregnant women to determine the type of autoantibodies that
were associated with severe, preeclampsia or spontaneous abortion.
SUBJECTS AND METHODS: We measured autoantibodies bound to prethrombin-1, pr
ethromhin-1, and fragment-1 by using an enzyme-linked immunosorbent assay (
ELISA) in 12 healthy nonpregnant women, 36 women with normal pregnancies, 2
8 pregnant women with severe preeclampsia, and 19 pregnant women who subseq
uently had spontaneous abortion.
RESULTS: Plasma samples in 10 (36%) of the 28 women with severe preeclampsi
a and 11 (58%) of the 19 women with spontaneous abortion were positive for
antiprothrombin antibodies as compared with 3 (9%) of the 36 women with nor
mal pregnancies. AU II of the positive samples from women who had spontaneo
us abortions were positive for antiprethrombin-1 antibody, but only 1 was p
ositive for antifragment-1 antibody. The mean ( +/- SD) titer of antiprethr
ombin-1 1 antibodies in patients with spontaneous abortion (36 +/- 9 U) was
higher than that in women with normal pregnancies (10 +/- 4 U; P < 0.01).
Antiprethrombin-1 antibody was detected in only 28 women with severe preecl
ampsia, whereas all 10 women with antiprethrombin antibodies were positive
for antifragment-1 antibody. The antifragment-1 antibody titer in patients
with severe pre eclampsia (49 +/- 15 U) was higher than in women with norma
l pregnancies(l3 +/- 6 U, P <0.01).
CONCLUSIONS: There is a strong and specific association between various typ
es of antiprothrombin antibodies with severe preeclampsia and spontaneous a
bortion. (C)2001 by Excerpta Medica, Inc.