Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys

Citation
P. Zheng et al., Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys, MOL REPROD, 58(3), 2001, pp. 348-355
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
MOLECULAR REPRODUCTION AND DEVELOPMENT
ISSN journal
1040-452X → ACNP
Volume
58
Issue
3
Year of publication
2001
Pages
348 - 355
Database
ISI
SICI code
1040-452X(200103)58:3<348:ESRFIV>2.0.ZU;2-U
Abstract
The energy substrates lactate, pyruvate, and glucose were evaluated for sup porting in vitro cytoplasmic maturation of rhesus monkey oocytes. A total o f 321 cumulus-oocyte complexes (COCs) aspirated from greater than or equal to 1000 mum diameter follicles of unstimulated adult monkeys were matured i n one of six media with various individual or combinations of energy substr ates: (1) mCMRL-1066 (control); (2) HECM-10 (containing 4.5 mM lactate); (3 ) HECM-10+0.2 mM pyruvate; (4) HECM-10+5.0 mM glucose; (5) HECM10+0.2 mM py ruvate+5.0 mM glucose; and (6) HECM-10 minus lactate+5.0 mM glucose. All me dia contained gonadotropins, oestradiol, and progesterone. Following matura tion, all mature oocytes were subjected to the same in vitro fertilization and embryo culture procedures. Oocytes matured in control medium or in trea tment groups 4 and 6 had the best morulae + blastocysts developmental respo nses (35, 36, and 32%, respectively, P < 0.05). HECM-10+0.2 mM pyruvate+5.0 mM glucose for COC maturation supported intermediate embryonic development (16% morulae + blastocysts). The lowest (P < 0.05) morula + blastocyst dev elopmental responses were obtained after maturation of COCs in HECM-10 and HECM10+0.2 mM pyruvate (4 and 6%, respectively). The COCs matured in glucos e-containing medium showed greater levels of cumulus expansion than those i n glucose-free medium. These results indicate that (a) glucose is both nece ssary and sufficient as the energy substrate for supporting optimal cytopla smic maturation in vitro of oocytes from unstimulated rhesus monkeys; (b) p yruvate suppresses the stimulatory effect of glucose on oocyte maturation; (c) glucose is involved in cumulus expansion; (d) cumulus expansion is not a reliable indicator of primate oocyte competence. (C) 2001 Wiley-Liss, Inc .