Low-density lipoprotein (LDL)-induced monocyte-endothelial cell adhesion, soluble cell adhesion molecules, and autoantibodies to oxidized-LDL in chronic renal failure patients on dialysis therapy

Citation
D. O'Byrne et al., Low-density lipoprotein (LDL)-induced monocyte-endothelial cell adhesion, soluble cell adhesion molecules, and autoantibodies to oxidized-LDL in chronic renal failure patients on dialysis therapy, METABOLISM, 50(2), 2001, pp. 207-215
Citations number
76
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
METABOLISM-CLINICAL AND EXPERIMENTAL
ISSN journal
0026-0495 → ACNP
Volume
50
Issue
2
Year of publication
2001
Pages
207 - 215
Database
ISI
SICI code
0026-0495(200102)50:2<207:LL(MCA>2.0.ZU;2-3
Abstract
Premature atherosclerosis is a major cause of morbidity and mortality in ch ronic renal failure patients undergoing dialysis, In this study, we compare d autoantibodies to oxidized low-density lipoprotein (OX-LDL), soluble cell adhesion molecules (CAMs), and the effect of both LDL and OX-LDL on monocy te endothelial cell adhesion in chronic renal failure patients on hemodialy sis (HD, n = 16) and peritoneal dialysis (PD, n = 17) compared with matched healthy control subjects (C, n = 17). In addition, we studied the effect o f supplementation with RRR-alpha-tocopherol (AT) 800 IU/d for 12 weeks on t he above measures. LDL and OX-LDL induced adhesion of U937 cells to culture d endothelium. soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble intracellular adhesion molecule-1 (sICAM-1), and soluble E-selectin (sf-se lectin); autoantibodies to OX-LDL and markers of lipid peroxidation were de termined before and after AT supplementation. Native LDL from PC patients i nduced greater monocyte-endothelial cell adhesion than LDL from C subjects (43.8% +/- 17.0% v 25.3% +/- 17.7%, respectively, P = .0028), OX-LDL from c hronic renal failure patients on both PD and HD stimulated greater adhesion than OX-LDL from the C subjects (68.0% +/- 18.5% and 57.6% +/- 15.1% v 40. 9% +/- 17.3%, respectively, P < .01); OX-LDL from PD patients induced great er adhesion than that from HD patients (P < .01). plasma methylglyoxal leve ls were significantly increased in both HD and PD groups, with higher level s in the HD group. Chronic renal failure patients on HD and PC also had hig her levels of plasma sVCAM-1 and sE-selectin than C subjects (P < .01), ind icating endothelial activation. Titers of autoantibodies to OX-LDL were not elevated in renal failure patients. Supplementation with AT 800 IU/d for 1 2 weeks, while resulting in significant enrichment with AT in LDL, did not have a significant effect on any of the parameters studied. This study make s the novel observation that the LDL of chronic renal failure patients on H D and PC appears to be potentially more atherogenic, since it induces great er monocyte-endothelial cell adhesion. Copyright (C) 2001 by W.B. Saunders Company.