Recognition and selective transport of nucleic acid components through molecularly imprinted polymeric membranes

Yoshikawa, M Izumi, J Guiver, MD Robertson, GP

M. Yoshikawa et al., Recognition and selective transport of nucleic acid components through molecularly imprinted polymeric membranes, MACRO MAT E, 286(1), 2001, pp. 52-59

39

INGLESE

Article

Organic Chemistry/Polymer Science

MACROMOLECULAR MATERIALS AND ENGINEERING

1438-7492 → ACNP

286

1

2001

52 - 59

ISI

1438-7492(200101)286:1<52:RASTON>2.0.ZU;2-C

Full Paper: To induce "molecular memory" in a membrane substrate at the sam e time that the membrane was prepared from its polymer solution, an alterna tive molecular imprinting technique was applied. Upon membrane formation, a "molecular memory" of the imprint molecule is retained by the formed membr ane that recognizes or favors interaction with print molecule analogues. In the present study, polystyrene resin bearing a tetrapeptide derivative, a derivative of natural polymer, and an entirely non-chiral synthetic polymer were adopted as candidate materials to form molecular recognition sites. 9 -Ethyladenine was adopted as a print molecule. These molecularly imprinted polymeric membranes recognized and adsorbed adenosine (As), which is an ana logue of the print molecule, in preference to guanosine (Gs) from As/Gs mix tures. However Gs was permeated in preference to As contrary to adsorption selectivity, possibly because of the relatively high affinity between As an d the membrane.