Enamel matrix derivative prolongs primary osteoblast growth

Citation
J. Jiang et al., Enamel matrix derivative prolongs primary osteoblast growth, J ENDODONT, 27(2), 2001, pp. 110-112
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Dentistry/Oral Surgery & Medicine
Journal title
JOURNAL OF ENDODONTICS
ISSN journal
0099-2399 → ACNP
Volume
27
Issue
2
Year of publication
2001
Pages
110 - 112
Database
ISI
SICI code
0099-2399(200102)27:2<110:EMDPPO>2.0.ZU;2-M
Abstract
Enamel matrix derivative (EMD) secreted by cells of the epithelial root she ath plays an important role in cementogenesis and periodontal tissue format ion. The mechanisms by which EMD influences cell function are not known. Th e purpose of this study was to determine the effect of EMD on cell growth o f primary mouse osteoblasts, Osteoblasts were digested from 6- to 8-day-old mouse calvaria and plated into 6-well cell culture plates at an initial de nsity of 5000 cells/cm(2). After 24-h incubation with Dulbecco's modified e agle medium (DMEM) supplemented with 10% fetal bovine serum, cells were inc ubated in three different groups of media: DMEM only as control, DMEM with 25 mug/ml EMD, and DMEM with 100 mug/ml EMD, At days 3, 7, 10, and 14, the total cell number per well was calculated, and cell morphology was examined . At each observation period the number of cells in the EMD groups was sign ificantly greater (ANOVA, p < 0.01) than that in the control group. EMD had a greater effect on osteoblast survivor in the higher concentration than i n the lower concentration, Furthermore normal morphology of the primary ost eoblasts was maintained in the EMD groups, These results suggest that EMD p rolongs primary osteoblast growth and may have an effect on osteoblasts dur ing periodontal regeneration.