Mismatch repair and microsatellite instability in esophageal cancer cells

Citation
N. Uchida et al., Mismatch repair and microsatellite instability in esophageal cancer cells, INT J CANC, 91(5), 2001, pp. 687-691
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF CANCER
ISSN journal
0020-7136 → ACNP
Volume
91
Issue
5
Year of publication
2001
Pages
687 - 691
Database
ISI
SICI code
0020-7136(20010301)91:5<687:MRAMII>2.0.ZU;2-L
Abstract
Using in vitro mismatch repair (MMR) assay, we have identified 3 of 22 esop hageal cancer cell lines exhibiting reduced MMR activity. By means of gel-s hift assay, decreased binding ability to GT mismatch and CA loop was observ ed in these 3 cell lines. However, we could not find any mutations in the h MSH2, hMSH3 and hMSH6 genes, the protein products of which exhibit mismatch binding activity in human cells. In addition, when using antibodies agains t 5 MMR-related proteins (hMSH2, hMSH1, hMSH6, hPMS2 and hMLH1), no aberran t expression was detected in any of them. When we examined 9 microsatellite loci in endogenous genomic DNA, these 3 esophageal cancer cell lines, defi cient in MMR, did not exhibit microsatellite instability. However, when we examined the repetitious sequence on exogenous plasmid DNA which was introd uced into these 3 esophageal cancer cells, the results suggested that MMR d eficiency in esophageal cancer cells could result in moderate instability o f the exogenous sequence. (C) 2001 Wiley-Liss, Inc.