Dopamine increases glial cell line-derived neurotrophic factor in human fetal astrocytes

Kinor, N Geffen, R Golomb, E Zinman, T Yadid, G

N. Kinor et al., Dopamine increases glial cell line-derived neurotrophic factor in human fetal astrocytes, GLIA, 33(2), 2001, pp. 143-150

32

INGLESE

Article

Neurosciences & Behavoir

GLIA

0894-1491 → ACNP

33

2

2001

143 - 150

ISI

0894-1491(200102)33:2<143:DIGCLN>2.0.ZU;2-V

The use of fetal astrocytes for gene delivery into brains with neurodegener ative diseases has been suggested. Therefore, the effects of neurotransmitt ers in the brain on such cells are of interest. The presence of D-1 (D-1A) receptors and the effect of dopamine on a fetal human astrocyte cell line ( SVG cells) in vitro were examined. SVG cells expressed D-1 (D-1A), but not D-5 (D-1B) receptors, as shown by RT-PCR. Exposure to dopamine, apomorphine , and the specific D-1 agonist, SKF-38393, increased glial-derived neurotro phic factor production of SVG cells, as well as intracellular free calcium. Exposure to the specific D-1 antagonist, SCH 23390, blocked these effects. Thus, if implanted into a brain region rich in dopamine, or if transfected with the tyrosine hydroxylase gene, fetal astrocytes may serve as paracrin e/autocrine cells capable of supplying critical growth factors to diseased brain tissue. (C) 2001 Wiley-Liss, Inc.