Design of an interface to allow microfluidic electrophoresis chips to drink from the fire hose of the external environment

Citation
S. Attiya et al., Design of an interface to allow microfluidic electrophoresis chips to drink from the fire hose of the external environment, ELECTROPHOR, 22(2), 2001, pp. 318-327
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
22
Issue
2
Year of publication
2001
Pages
318 - 327
Database
ISI
SICI code
0173-0835(200101)22:2<318:DOAITA>2.0.ZU;2-6
Abstract
An interface design is presented that facilitates automated sample introduc tion into an electrokinetic microchip, without perturbing the liquids withi n the microfluidic device. The design utilizes an interface flow channel wi th a volume flow resistance that is 0.54-4.1 x 10(6) times lower than the v olume flow resistance of the electrokinetic fluid manifold used for mixing, reaction, separation, and analysis. A channel, 300 mum deep, 1 mm wide and 15-20 mm long, was etched in glass substrates to create the sample introdu ction channel (SIC) for a manifold of electrokinetic flow channels in the r ange of 10-13 mum depth and 36-275 mum width. Volume flow rates of up to 1 mL/min were pumped through the SIC without perturbing the solutions within the electrokinetic channel manifold. Calculations support this observation, suggesting a leakage flow to electroosmotic flow ratio of 0.1:1% in the el ectrokinetic channels, arising from 66-700 muL/min pressure-driven flow rat es in the SIG. Peak heights for capillary electrophoresis separations in th e electrokinetic flow manifold showed no dependence on whether the SIC pump was on or off. On-chip mixing, reaction and separation of anti-ovalbumin a nd ovalbumin could be performed with good quantitative results, independent of the SIC pump operation. Reproducibility of injection performance, estim ated from peak height variations, ranged from 1.5-4%, depending upon the de vice design and the sample composition.