M. Cui et al., Efficient shoot regeneration from hairy roots of Antirrhinum majus L. transformed by the rol type MAT vector system, PL CELL REP, 20(1), 2001, pp. 55-59
Eleven independent GUS-positive hairy roots were induced by co-cultivation
of leaf explants of Antirrhinum majus L. with Agrobacterium tumefaciens str
ain GV2260 containing the rol type MAT vector pNPI702. The MAT vector pNPI7
02 possesses a GUS gene under the 35 S promoter and a removal element in wh
ich the 7.6-kb DNA fragments containing the rolA, B, C and D genes and reco
mbinase gene with a 35 S promoter are located between two directly oriented
recombination site sequences. A total of 326 adventitious shoots regenerat
ed from 11 independent hairy root lines cultured on 1/2MS medium without pl
ant growth regulators at 25 degreesC under a 16/8 fi (day/night) photoperio
d after 8 weeks of stock-culture of hairy roots and 4 weeks of culture of t
he green segments of hairy roots. Regenerated plants showed either a normal
or dwarf morphology. GUS activity was observed in the hairy roots and rege
nerated shoots. The presence of the GUS gene in the regenerated, morphologi
cally normal plants was confirmed by PCR analysis.