Efficient shoot regeneration from hairy roots of Antirrhinum majus L. transformed by the rol type MAT vector system

Citation
M. Cui et al., Efficient shoot regeneration from hairy roots of Antirrhinum majus L. transformed by the rol type MAT vector system, PL CELL REP, 20(1), 2001, pp. 55-59
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT CELL REPORTS
ISSN journal
0721-7714 → ACNP
Volume
20
Issue
1
Year of publication
2001
Pages
55 - 59
Database
ISI
SICI code
0721-7714(200101)20:1<55:ESRFHR>2.0.ZU;2-3
Abstract
Eleven independent GUS-positive hairy roots were induced by co-cultivation of leaf explants of Antirrhinum majus L. with Agrobacterium tumefaciens str ain GV2260 containing the rol type MAT vector pNPI702. The MAT vector pNPI7 02 possesses a GUS gene under the 35 S promoter and a removal element in wh ich the 7.6-kb DNA fragments containing the rolA, B, C and D genes and reco mbinase gene with a 35 S promoter are located between two directly oriented recombination site sequences. A total of 326 adventitious shoots regenerat ed from 11 independent hairy root lines cultured on 1/2MS medium without pl ant growth regulators at 25 degreesC under a 16/8 fi (day/night) photoperio d after 8 weeks of stock-culture of hairy roots and 4 weeks of culture of t he green segments of hairy roots. Regenerated plants showed either a normal or dwarf morphology. GUS activity was observed in the hairy roots and rege nerated shoots. The presence of the GUS gene in the regenerated, morphologi cally normal plants was confirmed by PCR analysis.