Role of HSP70i in regulation of biomaterial-induced activation of human monocytes-derived macrophages in culture

Citation
Rs. Bhardwaj et al., Role of HSP70i in regulation of biomaterial-induced activation of human monocytes-derived macrophages in culture, J MAT S-M M, 12(2), 2001, pp. 97-106
Citations number
66
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
ISSN journal
0957-4530 → ACNP
Volume
12
Issue
2
Year of publication
2001
Pages
97 - 106
Database
ISI
SICI code
0957-4530(200102)12:2<97:ROHIRO>2.0.ZU;2-I
Abstract
The functioning of an implant depends on the material properties and the wo und-healing process. The latter is led by an inflammatory reaction guided m ainly by monocyte/macrophage activity. This in vitro study investigated hum an monocytes/macrophages in culture from 2 h to 10 days on silicone, polyur ethane, teflon and TCPS. Analysis of cytokine release by ELISA showed that maturing macrophages have different capacities to produce cytokines TNF alp ha, IL10, IL8 and GM-CSF. The long culture-mature macrophages on all polyme rs produced comparable low levels of TNF alpha, IL10 and IL8. Monocytes/mac rophages on polyurethane and teflon, and those on silicone only in long cul ture-time produced high GM-CSF amounts, where as those on TCPS exhibited lo w levels of GM-CSF. FACS analysis revealed that HSP70i was highly inducible after short time culture yet this high level was maintained in long cultur e-mature macrophages on TCPS only, whereas on other polymers the mature mac rophages showed a high reduction in HSP70i level, which demonstrated a high stress-response by cells on TCPS. Accordingly, CLSM-analysis revealed low nuclear NF-kappaB in cells on TCPS and high nuclear NF-kappaB in mature mac rophages on silicone and polyurethane, showing a high cellular activation o n the latter two polymers. This corresponded also to the high mitochondrial activity by XTT metabolism displayed by the mature macrophages on polyuret hane greater than or equal to silicone > teflon > TCPS. These data show a c orrelation of (1) cytokines (TNF alpha, GM-CSF) and HSP70i, (2) NF-kappaB a nd HSP70i by monocytes/macrophages after contact with polymers. Thus, HSP70 i might be a useful molecular candidate for exploring biomaterial-induced i nflammatory reaction. (C) 2001 Kluwer Academic Publishers.