Transition from suspension to adherent growth is accompanied by tissue factor expression and matrix metalloproteinase secretion in a small cell lung cancer cell line

Citation
U. Salge et al., Transition from suspension to adherent growth is accompanied by tissue factor expression and matrix metalloproteinase secretion in a small cell lung cancer cell line, J CANC RES, 127(2), 2001, pp. 139-141
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
ISSN journal
0171-5216 → ACNP
Volume
127
Issue
2
Year of publication
2001
Pages
139 - 141
Database
ISI
SICI code
0171-5216(200102)127:2<139:TFSTAG>2.0.ZU;2-J
Abstract
Small cell lung cancer (SCLC) is a very malignant tumor known to grow aggre ssively and to metastasize early. It is well established that metastasis ge nerally involves both tumor cell adhesion and proteolytic degradation of th e extracellular matrix. However, SCLC cells cultured in vitro, such as the classic SCLC cell line NCI-H69, grow in floating aggregates and express onl y negligible proteolytic activity. In this report, we show that NCI-H69 cel ls can be selected for adherent growth. In contrast to parental suspension cells, the adherent cells were found to express tissue factor as well as ge latinolytic activity, attributable to matrix metalloproteinases 2 and 9. Su ch a switch of tumor cell characteristics, if it could occur in SCLC patien ts, might add to the understanding of the steps involved in the spreading o f this highly metastatic type of lung cancer.