Recombinant human extracellular matrix protein 1 inhibits alkaline phosphatase activity and mineralization of mouse embryonic metatarsals in vitro

Citation
Mml. Deckers et al., Recombinant human extracellular matrix protein 1 inhibits alkaline phosphatase activity and mineralization of mouse embryonic metatarsals in vitro, BONE, 28(1), 2001, pp. 14-20
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Endocrynology, Metabolism & Nutrition","da verificare
Journal title
BONE
ISSN journal
8756-3282 → ACNP
Volume
28
Issue
1
Year of publication
2001
Pages
14 - 20
Database
ISI
SICI code
8756-3282(200101)28:1<14:RHEMP1>2.0.ZU;2-H
Abstract
Two mRNAs are transcribed from the extracellular matrix protein 1 gene (Ecm 1): Ecm1a and an alternatively spliced Ecm1b. We studied Ecm1 mRNA expressi on and localization during endochondral bone formation and investigated the effect of recombinant human (rh) Ecm1a protein on organ cultures of embryo nic mouse metatarsals, Of the two transcripts, Ecm1a mRNA was predominantly expressed in fetal metacarpals from day 16 to 19 after gestation. Ecm1 exp ression was not found in 16- and 17-day-old metatarsals of which the perich ondrium was removed. In situ hybridization and immunohistochemistry demonst rated Ecm1 expression in the connective tissues surrounding the developing bones, but not in the cartilage. Biological effects of rhEcm1a protein on f etal metatarsal cultures were biphasic: at low concentrations, Ecm1a stimul ated alkaline phosphatase activity and had no effect on mineralization, whe reas at higher concentrations, Ecm1a dose dependently inhibited alkaline ph osphatase activity and mineralization, These results suggest that Ecm1a act s as a novel negative regulator of endochondral bone formation. (C) 2001 by Elsevier Science Inc, All rights reserved.